Triplex target sites of MEG3 RNA-chromatin interactions [version 1; referees: awaiting peer review]

Many long noncoding RNAs are bound to chromatin. MEG3 binds to multiple different genomic locations, containing GA-rich motifs, and form RNA-DNA triplex structures. In this work, we test whether the MEG3 binding sites are specific enough to be regulated by a particular lncRNA. We show that at least in the case of MEG3, a subset of the triplex target sites (TTS) is able to hybridize with various different RNAs almost irrespectively of their sequences. Nowadays, the role of chromatin bound RNAs in the formation of 3D chromatin structure is actively discussed. We speculate that such universal TTSs may contribute to establishing long-distance chromosomal contacts. Yulia Medvedeva ( ) Corresponding author: [email protected] : Conceptualization, Investigation, Methodology, Software, Validation, Visualization, Writing – Original Draft Preparation; Author roles: Antonov I : Conceptualization, Funding Acquisition, Project Administration, Supervision, Writing – Original Draft Preparation, Writing – Review Medvedeva Y & Editing No competing interests were disclosed. Competing interests: Antonov I and Medvedeva Y. How to cite this article: Triplex target sites of MEG3 RNA-chromatin interactions [version 1; referees: 2018, :76 (doi: ) awaiting peer review] F1000Research 7 10.12688/f1000research.13522.1 © 2018 Antonov I and Medvedeva Y. This is an open access article distributed under the terms of the Copyright: Creative Commons Attribution , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Data associated Licence with the article are available under the terms of the (CC0 1.0 Public domain dedication). Creative Commons Zero "No rights reserved" data waiver This work was supported by the Russian Science Foundation [grant 14-15-30002]. Grant information: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 17 Jan 2018, :76 (doi: ) First published: 7 10.12688/f1000research.13522.1 1,2